1XWQ
Structure Of A Cold-Adapted Family 8 Xylanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-15 |
| Detector | MAC Science DIP-2030B |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.991, 90.843, 98.139 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.880 |
| R-factor | 0.16131 |
| Rwork | 0.160 |
| R-free | 0.18731 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.019 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 34526 | |
| Completeness [%] | 90.3 | 82.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | MPD, sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
