1XWD
Crystal Structure of Human Follicle Stimulating Hormone Complexed with its Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.330, 66.938, 148.629 |
| Unit cell angles | 90.00, 99.13, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.920 |
| R-factor | 0.2213 |
| Rwork | 0.219 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fl7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.427 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.069 | 0.388 |
| Number of reflections | 25282 | |
| <I/σ(I)> | 12.4 | 3.4 |
| Completeness [%] | 98.6 | 97.7 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | PEG 3350, lithium sulfate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
