1XW8

X-ray structure of putative lactam utilization protein YBGL. Northeast Structural Genomics Consortium target ET90.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-09-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97909, 0.97928, 0.96742
Spacegroup name	C 1 2 1
Unit cell lengths	72.669, 37.208, 79.801
Unit cell angles	90.00, 93.97, 90.00

Refinement procedure

Resolution	25.860 - 2.000
R-factor	0.241
Rwork	0.241
R-free	0.26500
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	24348
<I/σ(I)>	30.5
Completeness [%]	86.7	90.6
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	0.2 M Sodium tartrate, 20% PEG 3350, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K