1XW4
Crystal Structure of Human Sulfiredoxin (Srx) in Complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-09 |
| Detector | RIGAKU |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.451, 68.451, 50.891 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.600 - 2.000 |
| R-factor | 0.22068 |
| Rwork | 0.218 |
| R-free | 0.27084 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xw3 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.828 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.640 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | |
| Number of reflections | 9562 | |
| <I/σ(I)> | 30.9 | 4.4 |
| Completeness [%] | 99.6 | 95.3 |
| Redundancy | 9.63 | 5.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.50 |
