1XVY
Crystal Structure of iron-free Serratia marcescens SfuA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-15 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.046, 65.392, 50.266 |
| Unit cell angles | 90.00, 111.90, 90.00 |
Refinement procedure
| Resolution | 46.630 - 1.740 |
| R-factor | 0.16495 |
| Rwork | 0.162 |
| R-free | 0.20927 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xvx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.304 |
| Data reduction software | d*TREK |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.880 | 1.800 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 27910 | |
| Completeness [%] | 99.2 | 95.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 273 | lithium chloride, PEG 6000, citric acid, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
