1XVW
Crystal Structure of AhpE from Mycobacterium tuberculosis, a 1-Cys peroxiredoxin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000, 0.97937, 0.97918, 0.95370 |
| Spacegroup name | I 4 |
| Unit cell lengths | 147.994, 147.994, 33.711 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.800 - 1.900 |
| Rwork | 0.180 |
| R-free | 0.21000 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 1.860 |
| High resolution limit [Å] | 1.870 | 1.800 |
| Number of reflections | 33679 | |
| <I/σ(I)> | 17.9 | 1.58 |
| Redundancy | 97.6 | 80.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 291 | 1.8M sodium malonate pH 5.0, 0.1M sodium acetate pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
