1XVO

Trypsin from Fusarium oxysporum at pH 6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-05
Detector	MARRESEARCH
Wavelength(s)	0.8126
Spacegroup name	P 1
Unit cell lengths	32.938, 37.086, 39.852
Unit cell angles	102.90, 104.48, 102.39

Refinement procedure

Resolution	25.000 - 0.840
R-factor	0.1082
Rwork	0.108
Starting model (for MR)	1pq5
RMSD bond length	0.014
RMSD bond angle	0.029
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	0.850
High resolution limit [Å]	0.840	0.840
Number of reflections	142228
<I/σ(I)>	11	3.1
Completeness [%]	91.6	57
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	sodium sulfate, sodium citrate, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 293K