1XVM

Trypsin from Fusarium oxysporum- room temperature to atomic resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	298
Detector technology	CCD
Collection date	2003-10-02
Detector	MARRESEARCH
Wavelength(s)	0.8030
Spacegroup name	P 1
Unit cell lengths	33.277, 37.037, 40.100
Unit cell angles	102.22, 103.93, 102.59

Refinement procedure

Resolution	10.000 - 1.100
R-factor	0.1439
Rwork	0.144
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pq5
RMSD bond length	0.022
RMSD bond angle	0.030
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.110
High resolution limit [Å]	1.100	1.100
Number of reflections	65556
<I/σ(I)>	6.9	2.6
Completeness [%]	93.0	92.4
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	ammonium sulfate, sodium citrate, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 293K