1XVJ

Crystal Structure Of Rat alpha-Parvalbumin D94S/G98E Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	173
Detector technology	IMAGE PLATE
Collection date	2003-03-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	30.719, 56.988, 56.493
Unit cell angles	90.00, 105.81, 90.00

Refinement procedure

Resolution	24.540 - 1.800
R-factor	0.19882
Rwork	0.197
R-free	0.23582
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.540	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.066	0.224
Number of reflections	15257
<I/σ(I)>	25	9.6
Completeness [%]	92.0	90
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1