1XVA

METHYLTRANSFERASE

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	MACSCIENCE
Spacegroup name	P 21 21 2
Unit cell lengths	86.400, 175.700, 45.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.196
Rwork	0.196
R-free	0.26200
RMSD bond length	0.014
RMSD bond angle	24.900 *
Data reduction software	KUAVST
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.200
Rmerge	0.068
Total number of observations	146394 *
Number of reflections	33778
<I/σ(I)>	3.4
Completeness [%]	93.5
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6	4 *	pH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	100 (mM)
2	1	reservoir	ammonium acetate	100 (mM)
3	1	reservoir		50 (mM)
4	1	reservoir	PEG4000	13 (%(w/v))
5	1	drop	protain	10 (mg/ml)