1XUF

TRYPSIN-BABIM-ZN+2, PH 8.2

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	54.800, 58.700, 67.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 1.900
R-factor	0.153
Rwork	0.153
R-free	0.22900
RMSD bond length	0.017
RMSD bond angle	26.500 *
Data reduction software	MSC
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.130
High resolution limit [Å]		2.040
Rmerge	0.085
Number of reflections	14155
Completeness [%]	57.0	51
Redundancy	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.2		SYNTHETIC MOTHER LIQUOR = 75% SATURATED MAGNESIUM SULFATE, 25 % 1.0 M TRIS ADJUSTED TO PH 8.2; SATURATED IN BABIM, 5.0 MM ZN+2. COMPLEX PRODUCED BY SOAKING TRYPSIN-BENZAMIDINE CO-CRYSTAL.