1XUB
Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.007 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 56.128, 56.128, 154.423 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.100 - 1.300 |
| R-factor | 0.13482 |
| Rwork | 0.133 |
| R-free | 0.15973 |
| Structure solution method | rigid body refinement of 1U1V |
| Starting model (for MR) | 1u1v |
| RMSD bond length | 0.035 |
| RMSD bond angle | 2.714 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.400 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 63575 | |
| <I/σ(I)> | 15.2 | 4.6 |
| Completeness [%] | 91.7 | 69.5 |
| Redundancy | 5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 292 | 11% (w/v) PEG 3350, 0.1M Na Citrate, 0.2M (NH4)2 Sulfate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
