1XTY
Crystal structure of Sulfolobus solfataricus peptidyl-tRNA hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.816, 67.232, 85.352 |
| Unit cell angles | 90.00, 95.08, 90.00 |
Refinement procedure
| Resolution | 46.600 - 1.800 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.22400 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.123 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 48937 | |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 5.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 2.8 | 297 | 0.8Ml SO4, 1.6% PEG 8000, pH 2.8, VAPOR DIFFUSION, temperature 297K |
