1XTV

Sulfolobus solfataricus uracil phosphoribosyltransferase with uridine 5'-monophosphate (UMP) bound to half of the subunits

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-10
Detector	MARRESEARCH
Wavelength(s)	1.133
Spacegroup name	P 1
Unit cell lengths	68.100, 73.570, 115.100
Unit cell angles	85.82, 85.28, 62.46

Refinement procedure

Resolution	25.000 - 2.600
Rwork	0.227
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Sulfolobus solfataricus UPRTase PDB ID 1XTT
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	55389
<I/σ(I)>	6.8	1.3
Completeness [%]	91.4	79.7
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K