1XTS

Structure of small GTPase human Rheb in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-05-26
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	102.410, 98.390, 47.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.800 - 2.800
Rwork	0.231
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1KAO 1gua
RMSD bond length	0.009
RMSD bond angle	1.400
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.800	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.156	0.333
Number of reflections	6145
<I/σ(I)>	3.9	1.6
Completeness [%]	98.8	98.5
Redundancy	3.5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	277	ammonium dihydrogenphosphate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K