1XTJ
structure of human UAP56 in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-13 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.988, 78.201, 63.192 |
| Unit cell angles | 90.00, 103.42, 90.00 |
Refinement procedure
| Resolution | 32.700 - 2.700 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xti |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.298 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.700 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 9164 | |
| Completeness [%] | 97.0 | 94.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 290 | MPD Tris MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 7.50 |
