1XT0
The Structure of N-terminal Sec7 domain of RalF
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 108 |
| Detector technology | CCD |
| Collection date | 2003-08-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.195, 43.244, 109.132 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.160 |
| Rwork | 0.215 |
| R-free | 0.27150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xsz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.066 | 0.115 |
| Number of reflections | 17589 | |
| <I/σ(I)> | 12.1 | 6.1 |
| Completeness [%] | 91.0 | 92.6 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | PEG2K, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
