1XSQ
Crystal structure of ureidoglycolate hydrolase from E.coli. Northeast Structural Genomics Consortium target ET81.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.194, 45.273, 51.801 |
| Unit cell angles | 100.70, 99.84, 117.19 |
Refinement procedure
| Resolution | 24.340 - 1.600 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.25700 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.036 | 0.171 |
| Number of reflections | 80698 | |
| <I/σ(I)> | 42.7 | 5.9 |
| Completeness [%] | 93.1 | 77.6 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Na-citrate, Na-formate, PEG-3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
