1XSM
PROTEIN R2 OF RIBONUCLEOTIDE REDUCTASE FROM MOUSE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-02-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.078, 108.932, 92.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rib |
| RMSD bond length | 0.018 |
| RMSD bond angle | 28.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.290 |
| Rmerge | 0.104 | 0.300 * |
| Total number of observations | 144719 * | |
| Number of reflections | 17131 | |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 95.8 * | 70.9 |
| Redundancy | 8.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.7 | 20 * | Nielsen, B.B., (1995) FEBS Letters, 373, 310. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | 0.8-1.0 (M) | ||
| 3 | 1 | drop | acetate | 50 (mM) | |
| 4 | 1 | reservoir | 0.8-1.0 (M) | ||
| 5 | 1 | reservoir | acetate | 50 (mM) |
