1XSJ
Structure of a Family 31 alpha glycosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.532, 224.442, 154.117 |
| Unit cell angles | 90.00, 101.75, 90.00 |
Refinement procedure
| Resolution | 29.980 - 2.100 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xsi |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 0.104 |
| Number of reflections | 299802 | |
| <I/σ(I)> | 10.4 | 5.4 |
| Completeness [%] | 93.2 | 88.1 |
| Redundancy | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100 mM Tris, 15% PEG 1500, 5% MPD, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
