1XS5
The Crystal Structure of Lipoprotein Tp32 from Treponema pallidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-23 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.248, 46.404, 55.217 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.500 - 1.850 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.23200 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.500 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 21397 | |
| <I/σ(I)> | 17 | 1.9 |
| Completeness [%] | 98.1 | 87.7 |
| Redundancy | 4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | succinic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
