1XS0
Structure of the E. coli Ivy protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97917, 0.97941, 0.97393 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.339, 46.962, 88.044 |
| Unit cell angles | 90.00, 89.95, 90.00 |
Refinement procedure
| Resolution | 14.520 - 1.580 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.26000 |
| Structure solution method | MAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.700 | 1.670 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 44577 | |
| <I/σ(I)> | 3.1 | |
| Completeness [%] | 98.1 | 98.1 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 293 | 1.4M sodium citrate, 8% PEG 8000, 20mM TRIS, 50mM NaCl, pH 6.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
