1XRY

Crystal structure of Aeromonas proteolytica aminopeptidase in complex with bestatin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1992-12-14
Detector	XENTRONICS
Wavelength(s)	1.5418
Spacegroup name	P 61 2 2
Unit cell lengths	107.390, 107.390, 103.060
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.690 - 2.100
R-factor	0.168
Rwork	0.164
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1amp
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	X-GEN
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	CNX (2002)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.700	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	20836
<I/σ(I)>	31.1
Completeness [%]	99.1	94.7
Redundancy	15.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	NaCl, KSCN, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K