1XRX
Crystal structure of a DNA-binding protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97938, 0.96859, 0.98241 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 112.179, 112.179, 62.021 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.710 - 2.150 |
Rwork | 0.222 |
R-free | 0.23700 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.900 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.076 | 0.460 |
Number of reflections | 24398 | |
<I/σ(I)> | 26.1 | 2.1 |
Completeness [%] | 98.5 | 84.6 |
Redundancy | 15.7 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | MPD, calcium chloride, TRIS, isopropanol, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | MPD, calcium chloride, TRIS, isopropanol, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | MPD, calcium chloride, TRIS, isopropanol, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |