1XRU
Crystal Structure of 5-keto-4-deoxyuronate Isomerase from Eschericia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.980122, 0.979634, 0.979538 |
| Spacegroup name | H 3 |
| Unit cell lengths | 102.972, 102.972, 176.862 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.970 - 1.940 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.19100 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 51573 | |
| <I/σ(I)> | 9 | 4.8 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | PEG 400, calcium chloride, HEPES, pH 7, VAPOR DIFFUSION, temperature 277K |
