1XRG
Conserved hypothetical protein from Clostridium thermocellum Cth-2968
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.969 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.436, 80.436, 137.202 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.897 - 2.200 |
| R-factor | 0.19353 |
| Rwork | 0.193 |
| R-free | 0.22480 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.352 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.06) |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.072 | 0.048 | 0.169 |
| Total number of observations | 3241 | 3022 | |
| Number of reflections | 30510 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 291 | 30 v/v% PEG 400, 0.1M HEPES, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
