1XQR
Crystal structure of the HspBP1 core domain
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.680, 84.286, 89.945 |
| Unit cell angles | 90.00, 96.00, 90.00 |
Refinement procedure
| Resolution | 38.070 - 2.100 |
| R-factor | 0.21299 |
| Rwork | 0.211 |
| R-free | 0.24880 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.706 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 33305 | |
| <I/σ(I)> | 5.9 | 2.8 |
| Completeness [%] | 98.9 | 99.2 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 293 | PEGMME2000, Potassium Acetate, Glycerol, iso-propanol, EDTA, TCEP, Tris-HCl, pH 8.5, microbatch, temperature 293K |
