1XQA
Structure of a possible Glyoxalase from Bacillus cereus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2004-05-30 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.980141 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.535, 61.616, 78.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.450 - 1.800 |
| R-factor | 0.19155 |
| Rwork | 0.189 |
| R-free | 0.23552 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.257 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.060 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.810 |
| Number of reflections | 19959 | |
| <I/σ(I)> | 6.5 | 2.5 |
| Completeness [%] | 98.9 | 94.1 |
| Redundancy | 5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG MME 550, NaCl, BisTris, glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
