1XPX

Structural basis of prospero-DNA interaction; implications for transcription regulation in developing cells

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 43 21 2
Unit cell lengths	44.708, 44.708, 226.661
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.217
Rwork	0.217
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	6354
<I/σ(I)>	33
Completeness [%]	90.0	90

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5		pH 5.00