1XPP

Crystal Structure of TA1416,DNA-directed RNA polymerase subunit L, from Thermoplasma acidophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-19
Detector	SBC-2
Wavelength(s)	0.9793
Spacegroup name	P 1 2 1
Unit cell lengths	59.700, 69.424, 66.293
Unit cell angles	90.00, 116.77, 90.00

Refinement procedure

Resolution	23.900 - 1.600
R-factor	0.22355
Rwork	0.219
R-free	0.26351
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.417
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.900	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.069	0.347
Number of reflections	60955
<I/σ(I)>	8.6	2.9
Completeness [%]	95.2	91.7
Redundancy	4.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	potassium thiocyanate, PEG MME 2000, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	potassium thiocyanate, PEG MME 2000, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K