1XPJ
Crystal Structure of MCSG Target APC26283 from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 99.8 |
| Detector technology | CCD |
| Collection date | 2004-08-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.729, 52.888, 72.215 |
| Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.25300 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.191 |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.060 | 0.317 |
| Number of reflections | 30494 | |
| <I/σ(I)> | 18.68 | 4.98 |
| Completeness [%] | 1.0 | 0.9 |
| Redundancy | 7.44 | 5.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 295 | 0.2M di-Ammonium Tartrate, 20% w/v PEG 3350, HgCl2, 5% PEG 4000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 6.60 |
