1XPI
Crystal structure of the catalytic domain of E. coli pseudouridine synthase RluC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 97.307, 97.307, 86.237 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.18805 |
| Rwork | 0.185 |
| R-free | 0.25372 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1V9K (molecule A) |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.906 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.067 | 0.441 |
| Number of reflections | 24381 | |
| <I/σ(I)> | 38.4 | 6.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | Bicine, sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
