1XP3
Crystal Structure of Endonuclease IV (BA4508) from Bacillus anthracis at 2.57A Resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.886, 55.390, 64.972 |
| Unit cell angles | 90.00, 89.43, 90.00 |
Refinement procedure
| Resolution | 22.830 - 2.570 |
| R-factor | 0.15478 |
| Rwork | 0.151 |
| R-free | 0.22808 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qtw |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.649 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.660 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.061 | 0.242 |
| Number of reflections | 10633 | |
| <I/σ(I)> | 14 | 4 |
| Completeness [%] | 93.7 | 86.6 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG 5000, ammonium sulfate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
