1XOV
The crystal structure of the listeria monocytogenes bacteriophage PSA endolysin PlyPSA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 90.586, 90.586, 213.667 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.800 |
| R-factor | 0.17164 |
| Rwork | 0.170 |
| R-free | 0.19831 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.591 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.700 | |
| Number of reflections | 48940 | |
| <I/σ(I)> | 5.34 | 1.05 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 18.79 | 17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 100mM hepes, 0.2M (nh4)2so4, 25% peg 3350, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
