1XOU
Crystal structure of the CesA-EspA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.422, 72.383, 95.679 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.730 - 2.800 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.26500 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.730 | 2.940 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.123 | 0.507 |
| Number of reflections | 6480 | |
| <I/σ(I)> | 21.5 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | PEG 1500, Tris, pH 8.5, VAPOR DIFFUSION, temperature 293K |
