1XOK

crystal structure of alfalfa mosaic virus RNA 3'UTR in complex with coat protein N terminal peptide

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-10
Detector	CUSTOM-MADE
Wavelength(s)	0.91942, 0.91997, 0.92181
Spacegroup name	C 2 2 21
Unit cell lengths	50.100, 123.000, 53.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.251
Rwork	0.249
R-free	0.26888
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	2.118
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	3.190
High resolution limit [Å]	3.000	3.000
Number of reflections	3487
<I/σ(I)>	25	4.4
Completeness [%]	99.4	98.1
Redundancy	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	2.45M ammonium sulfate, 0.01M magnesium acetate, 0.01M mes, 0.001M samarium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	2.45M ammonium sulfate, 0.01M magnesium acetate, 0.01M mes, 0.001M samarium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	2.45M ammonium sulfate, 0.01M magnesium acetate, 0.01M mes, 0.001M samarium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K