1XO6

Acyl-CoA Carboxylase Beta Subunit from S. coelicolor (PccB), apo form #3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-09
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	86.773, 178.954, 296.358
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.410 - 2.200
R-factor	0.2091
Rwork	0.209
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.410	2.260
High resolution limit [Å]	2.200	2.200
Rmerge		0.532
Number of reflections	201135
<I/σ(I)>	11.2	2.2
Completeness [%]	92.4	92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.22 M Mg formate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K