1XO0

High resolution structure of the holliday junction intermediate in cre-loxp site-specific recombination

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9188
Spacegroup name	C 2 2 21
Unit cell lengths	107.400, 123.000, 180.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.000 - 2.000
Rwork	0.219
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1crx
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000	2.090
High resolution limit [Å]	2.000	2.000
Number of reflections	58491
Completeness [%]	96.4	84.2
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	20mM ACETATE BUFFER PH 5.0 40%MPD 20mM CACL2. , VAPOR DIFFUSION, HANGING DROP, temperature 291K