1XNV
Acyl-CoA Carboxylase Beta Subunit from S. coelicolor (PccB), apo form #1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 63 |
| Unit cell lengths | 166.942, 166.942, 80.494 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.250 - 2.300 |
| R-factor | 0.1891 |
| Rwork | 0.189 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model of PccB |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.250 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 111570 | |
| <I/σ(I)> | 19 | 3.43 |
| Completeness [%] | 100.0 | 93.6 |
| Redundancy | 7.9 | 7.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 1.4 M sodium citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
