1XMV
E. coli RecA in complex with MgADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-31 |
| Wavelength(s) | 1.033201 |
| Spacegroup name | P 61 |
| Unit cell lengths | 100.509, 100.509, 82.227 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.140 - 1.900 |
| Rwork | 0.202 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2reb |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.140 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.053 | 0.533 |
| Number of reflections | 36969 | |
| <I/σ(I)> | 62.4 | 6.5 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 10.1 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | ethylene glycol, MES, Tris-HCl, DTT, NaF, MgCl2, ADP, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
