1XMT
X-ray structure of gene product from arabidopsis thaliana at1g77540
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.926676, 0.926676, 0.919801, 0.919528, 0.91302 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.349, 60.601, 29.424 |
| Unit cell angles | 90.00, 91.50, 90.00 |
Refinement procedure
| Resolution | 30.303 - 1.150 |
| R-factor | 0.149 |
| Rwork | 0.148 |
| R-free | 0.17030 |
| Structure solution method | MAD (BROMIDE PHASING) |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.022 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.290 | 50.000 | 1.180 |
| High resolution limit [Å] | 1.150 | 2.840 | 1.150 |
| Rmerge | 0.039 | 0.030 | 0.345 |
| Total number of observations | 2294 | 1606 | |
| Number of reflections | 32713 | ||
| <I/σ(I)> | 16.5 | 2.22 | |
| Completeness [%] | 95.9 | 98.5 | 70.7 |
| Redundancy | 3.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PROTEIN 10 MG/ML, 29% MEPEG 5K, 0.100 M SODIUM CITRATE, 0.100 M PIPES, CRYOPROTRECTED CRYSTAL SOAKED IN 1 M SODIUM BROMIDE FOR CIRCA 40 SECONDS, pH 6.5, Vapor diffusion, hanging drop, temperature 277 KK |
