1XMS
E. coli RecA in complex with MnAMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 61 |
Unit cell lengths | 100.700, 100.700, 81.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.490 - 2.100 |
Rwork | 0.223 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2reb |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.490 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.050 | 0.329 |
Number of reflections | 26173 | |
<I/σ(I)> | 18.4 | 2.9 |
Completeness [%] | 94.8 | 66.3 |
Redundancy | 4.7 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | ethylene glycol, MES, Tris-HCl, AMP-PNP, MnCl2, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |