1XMK

The Crystal structure of the Zb domain from the RNA editing enzyme ADAR1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-22
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	35.557, 43.526, 45.471
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 0.970
R-factor	0.145
R-free	0.18300
Structure solution method	MIR
RMSD bond length	0.018
RMSD bond angle	0.035
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	SHELX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.920	1.020
High resolution limit [Å]	0.970	0.970
Number of reflections	41638
<I/σ(I)>	5.2
Completeness [%]	99.2	94.7
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9	312	PEG1000, Cadmium Chloride, Nickel Chloride, Tris, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 312K, pH 9.00