1XMA
Structure of a transcriptional regulator from Clostridium thermocellum Cth-833
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU SATURN 92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.483, 52.160, 51.076 |
| Unit cell angles | 90.00, 92.46, 90.00 |
Refinement procedure
| Resolution | 18.239 - 2.301 |
| R-factor | 0.20853 |
| Rwork | 0.205 |
| R-free | 0.26676 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.06) |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.730 | 2.200 |
| Rmerge | 0.049 | 0.044 | 0.151 |
| Number of reflections | 10985 | ||
| Completeness [%] | 100 | 43.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 291 | 8.5% v/v isopropanol, 10% w/v PEG 4000, 15% v/v glycerol, 0.085M TRIS, modified microbatch, temperature 291K |
