1XM2
Crystal structure of Human PRL-1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-16 |
| Detector | MACSCIENCE |
| Wavelength(s) | 0.979, 0.9792, 0.9794, 0.9716 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.290, 84.760, 122.180 |
| Unit cell angles | 90.00, 99.79, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.700 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.29600 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.370 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.079 | 0.260 |
| Number of reflections | 31124 | |
| <I/σ(I)> | 7.2 | 2.9 |
| Completeness [%] | 94.5 | 95.4 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG 4K, Sodium Acetate, AMS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
