1XLO
Structure of reduced C73S/C85S putidaredoxin, a [2Fe-2S] ferredoxin from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.098, 75.097, 108.108 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.800 - 1.840 |
| R-factor | 0.2381 |
| Rwork | 0.230 |
| R-free | 0.23750 |
| Structure solution method | rigid body refinement |
| Starting model (for MR) | 1oqq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.840 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.097 | 0.498 |
| Number of reflections | 17739 | |
| <I/σ(I)> | 16.6 | 1.6 |
| Completeness [%] | 98.0 | 93.8 |
| Redundancy | 3.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | Ammonium sulfate, sodium citrate, sodium/potassium phosphate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
