1XL8

Crystal structure of mouse carnitine octanoyltransferase in complex with octanoylcarnitine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9790
Spacegroup name	H 3
Unit cell lengths	163.610, 163.610, 159.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.090 - 2.200
R-factor	0.199
Rwork	0.199
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xl7
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	COMO
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	80628
Completeness [%]	99.9	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	277	100mM Hepes, 62%v/vMPD, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K