1XKZ
Crystal structure of the acylated beta-lactam sensor domain of Blar1 from S. aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-27 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.205, 109.791, 91.605 |
Unit cell angles | 90.00, 106.13, 90.00 |
Refinement procedure
Resolution | 69.000 - 1.750 |
R-factor | 0.209 |
Rwork | 0.205 |
R-free | 0.23400 |
Structure solution method | rigid body refinement |
Starting model (for MR) | SAD structure model solved with selenomethionylated BlaR protein |
RMSD bond length | 0.021 |
RMSD bond angle | 1.870 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.000 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 110752 | |
<I/σ(I)> | 6 | 2.7 |
Completeness [%] | 98.0 | 98 |
Redundancy | 4.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | PEG MME 5000, lithium sulfate, nonyl-D-glucoside, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |