1XKU
Crystal structure of the dimeric protein core of decorin, the archetypal small leucine-rich repeat proteoglycan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-25 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.780, 124.145, 129.609 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 2.150 |
| R-factor | 0.19093 |
| Rwork | 0.190 |
| R-free | 0.21747 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.719 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.200 | 2.280 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 24593 | |
| <I/σ(I)> | 8.7 | 2.1 |
| Completeness [%] | 98.7 | 93.7 |
| Redundancy | 10.7 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.75 | 293 | PEG 400, TRIS, OCTYL-BETA-D-GLUCOPYRANOSIDE, SODIUM AZIDE, pH 7.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
