1XKJ
BACTERIAL LUCIFERASE BETA2 HOMODIMER
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-10-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 112.400, 112.400, 153.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | LUCIFERASE AB HETERODIMER (PDB ENTRY 1BRL) |
| RMSD bond length | 0.013 |
| RMSD bond angle | 23.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.843) |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | |
| High resolution limit [Å] | 2.500 | 2.500 * |
| Rmerge | 0.088 | |
| Total number of observations | 245416 * | |
| Number of reflections | 32225 | |
| Completeness [%] | 93.0 | 78 * |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 4 * | SITTING DROP, 277 KELVIN, 12% PEG 4000, 17-21% ISOPROPANOL, 0.006M DTT, 0.1M CITRATE PH 6.2. 50 MG/ML PROTEIN., vapor diffusion - sitting drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 12 (%) | |
| 2 | 1 | reservoir | 2-propanol | 17-21 (%) | |
| 3 | 1 | reservoir | DTT | 6 (mM) | |
| 4 | 1 | reservoir | citrate | 0.1 (M) |
